In 1998 Wengel
and co-workers described constrained nucleotides. They described it as a
original class of conformationally limited oligonucleotide analogues. It is
assumed as bicyclic nucleic acid where a ribonucleoside is closely connected among
the 2'-oxygen and the 4'-carbon atoms all the way through a
methylene unit.
Biosyn is competent enough
to offer its valuable customers, various customized solutions for custom peptide synthesis,
custom RNA synthesis, custom DNA synthesis, custom polyclonal antibody
production, DNA real-time PCR products, DNA micro-array services, molecular
diagnostic detection kits, DNA micro-array services and a wide range of custom
DNA-based consumables and analytical kits since it is a market leader in
genomic research and proteomic research products.
As you know Bio-synthesis
is exclusively located and able to synthesize long RNA
segment with a bit longer in length, speedy turnaround period and one of the
best quality.
Oligonucleotides enclosed
nucleotide show unparalleled thermal stability towards balancing RNA2 and DNA,
which lets excellent unequal discrimination. Actually, the high binding similarity
of constrained nucleotide oligo is prescribed for the purpose of any of the
hydration assay that needs high specificity and reproducibility like:
Most of the constrained
nucleotide oligo synthesis includes:
Ø Preparation of mRNA sample
Ø PCR primes and molecular beacons
Ø Allele précised PNA
Ø SNP genotyping
Ø Analysis of DNA MicroArray expression
Ø Tagged probes
Ø In situ hybridization investigation
Ø Capacities of multi scale
Ø Desalted, deprotected and prepared to use.
Ø Quality analysis by MALDI-TOF Mass spectrometry, PAGE analysis and
HPLC
Ø High throughput capacity
speedy capacity.
As a consequence of these
important characteristics, the actual use of constrained nucleotide-altered
oligo in antisense drug development is these days coming under investigation4
and, freshly, the therapeutic potential of constrained nucleotide has been evaluated.
There is an contribution from BSI that is constrained nucleotide synthesis with
great flexibility.
Quality that is fully guaranteed
Here at Bio-synthesis, we are committed to provide on our
reputation for one of the best quality nucleic acid synthesis. As an important
part of our long lasting assurance to the best quality, we implement the
following standards for the assurance of quality:
DNA, RNA, PNA, siRNA, and all nucleic acid synthesis
products are evaluated by mass spectrometry and quantified at least two times
by uv spectrophotometry (unless no post-synthesis processing is demanded)
All standard RNA, DNA, PNA and siRNA synthesis requires
MALDI-TOFMS.
ESI-MS for all extended or highly customized
oligonucleotides.
PAGE, HPLC, RNase Free HPLC and PAGE for cleansing of RNA
and DNA products.
CGE or UPLC for quality
evaluation when suitable.
Quality checking methods that are fully customized and
documentation are available upon request.
Assured Quality with excellent features
At Bio-synthesis, we feel very proud on our old and good
reputation for high and better quality synthesis of nucleic acid.
As an important part of long-standing promise to quality, we
use the following standards for excellence pledge:
TOFMS-MALDI for every standard RNA, DNA, siRNA, and PNA
synthesis
MS-ESI for every longer or highly customized
oligonucleotides.
For more information on Peptide Synthesis, Amino
acid analysis, Long RNA, Please
Visit : Oligo
Synthesis